a301 913a Search Results


anti nelf e antibody  (Bethyl)
92
Bethyl anti nelf e antibody
Curve fitting data for <t> NELF </t> RNA and DNA binding as determined by fluorescence anisotropy. Fluorescence anisotropy data found in <xref ref-type= Figures 5 – 7 were fit with a single site binding model where possible (Materials and methods). Apparent disassociation constants (K d,app ), R 2 , and Bmax (maximum anisotropy) values with error where applicable for the 60% ssRNA and DNA substrate as well as the TAR RNA are shown. NA means fitting was not applicable. DOI: http://dx.doi.org/10.7554/eLife.14981.016" width="250" height="auto" />
Anti Nelf E Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a301+913a/NELFE+Antibody/pmc04940160-556-27-29
Average 92 stars, based on 1 article reviews
anti nelf e antibody - by Bioz Stars, 2026-09
92/100 stars
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Curve fitting data for NELF RNA and DNA binding as determined by fluorescence anisotropy. Fluorescence anisotropy data found in <xref ref-type= Figures 5 – 7 were fit with a single site binding model where possible (Materials and methods). Apparent disassociation constants (K d,app ), R 2 , and Bmax (maximum anisotropy) values with error where applicable for the 60% ssRNA and DNA substrate as well as the TAR RNA are shown. NA means fitting was not applicable. DOI: http://dx.doi.org/10.7554/eLife.14981.016" width="100%" height="100%">

Journal: eLife

Article Title: Architecture and RNA binding of the human negative elongation factor

doi: 10.7554/eLife.14981

Figure Lengend Snippet: Curve fitting data for NELF RNA and DNA binding as determined by fluorescence anisotropy. Fluorescence anisotropy data found in Figures 5 – 7 were fit with a single site binding model where possible (Materials and methods). Apparent disassociation constants (K d,app ), R 2 , and Bmax (maximum anisotropy) values with error where applicable for the 60% ssRNA and DNA substrate as well as the TAR RNA are shown. NA means fitting was not applicable. DOI: http://dx.doi.org/10.7554/eLife.14981.016

Article Snippet: For endogenous NELF-A and NELF-E immunoprecipitation experiments, 30 mg of total protein was incubated with 70 μg of anti-NELF-A antibody (Santa Cruz, sc-23599) or 50 μg of anti-NELF-E antibody (Bethyl Laboratories, A301-914A) conjugated to Protein G Dynabeads (Invitrogen, B00262).

Techniques: Binding Assay, Fluorescence, Construct, Mutagenesis